4 clinical applications of bone tissue engineering
liver stem cells methods and protocols
... Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore MARI DEZAWA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, ... portal vasculature if repeated transfusions of large number of cells are required, further increasing the risks of bleeding, infection, and loss of viability of transplanted cells After infusion into ... hepatocytes and the lack of such systems has limited the understanding of pathogenesis of these diseases, development of drugs, and testing for resistance 2 Clinical Uses of Liver Stem Cells 21...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... Downregulation of HIF-1a decreased the enhanced PI of NPCs induced by hypoxia (A) Cytometry analysis indicates that the PI of NPCs was decreased by downregulation of HIF-1a (B) Graph of the PI of NPCs ... growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells [12] Therefore, ... Animal Care and Use Committee (IACUC) of the Academy of Military Medical Science gave consent for the use of rats in all of the experiments Isolation and culture of NPCs Cells derived from Wistar...
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báo cáo hóa học:" Circulating endothelial progenitor cells: a new approach to anti-aging medicine?" docx
... hundreds of years as a starter culture for the production of sour dough bread and provides for its characteristic sour flavor Extract of green tea, extract of goji berries, and extract of the root of ... tissue insult is acute respiratory distress syndrome (ARDS), in which respiratory failure often occurs as a result of disruption of the alveolarcapillary membrane, which causes accumulation of ... severity of illness [61] From an interventional perspective, transplantation of EPC into a rabbit model of acute lung injury resulted in reduction of leukocytic infiltrates and preservation of pulmonary...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... plated at a concentration of 20000 cells per well in 96 well plates After days of culture, level of cellular ATP was quantified by bio-luminescence The ratio of average values of ATP in growth factor ... existence of various subsets of cells with EPC potential residing outside of the CD34+ KDR+ fraction Further studies are required to elucidate functional importance of the variable kinetics of mobilization, ... composed of a combination of blueberries, green tea extract, carnosine, and vitamin D3 In vitro activity on proliferation of human bone marrow cells was assessed, in which a 60% enhancement of growth...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong to the family of mesenchymal ... increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of MSCs in ... of the hepatic artery, and the presence of any vascular occlusion or changes in the flow patterns We will monitor lung toxicity by assessing the necessity of reintubation and the occurrence of...
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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx
... with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong to the family of mesenchymal ... increased risk of infections or renal dysfunction In view of the synergistic interplay of MSCs with mycophenolic acid, and because CNIs have been shown to abolish the beneficial effect of MSCs in ... of the hepatic artery, and the presence of any vascular occlusion or changes in the flow patterns We will monitor lung toxicity by assessing the necessity of reintubation and the occurrence of...
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Transcoronary Transplantation of Progenitor Cells after Myocardial Infarction ppt
... previously was followed by the administration of 7500 to 10,000 U of heparin and (in 89% of the cell-treated patients) a bolus of abciximab (0.25 mg per kilogram of body weight) Cells were infused into ... in because of inadequate contrast opacification, in because of ventricular extrasystoles, and in because of the patients’ refusal to undergo invasive follow-up Thus, a total of 72 of 81 serial ... board of directors of and holding equity in Aetna From the Department of Health Care Policy, Harvard Medical School; and the Department of Health Policy and Management, Harvard School of Public...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... low-dose intravenous applications of cisplatin [33] and 8-10 µM for similar applications of BCNU [34,35], and can be up to two orders of magnitude or greater in high-dose applications [36-40] ... great as for many of the more widely recognized neurological syndromes Despite the clear evidence of the neurotoxicity of at least some forms of chemotherapy, studies on the effects of chemotherapeutic ... up to 70% of individuals, usually with a delayed onset of several months after treatment [1,2] Even if examination of all cancers were to lower the frequency of these problems to 25% of the lower...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... terms of the Ethics Committee of the University of Ulm The age of the donors ranged from 55 to 89 years (mean 74 years) The diagnosis was based on clinical and radiological criteria None of the ... After 14 days of differentiation, the gene expression profile of osteoblast markers was investigated For OC cells and bone marrow MPCs we detected a strong signal for expression of genes for all ... recapitulation of development such as expression of procollagen type IIA [29] Possibly, the activation of MPCs may contribute to the observed expression of this alternative splice variant A misguiding of...
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Báo cáo y học: "It’s all in the blood: circulating endothelial progenitor cells link synovial vascularity with cardiovascular mortality in rheumatoid arthritis" docx
... comparable levels of adhesion to unstimulated endothelial cells or matrix proteins such as laminin [8] What is the relevance of these findings in terms of our understanding of RA? Increased angiogenesis ... observations of reduced peripheral blood EPC in RA However, the corollary of augmented synovial tissue EPC paralleled by reduced circulating EPC might also be an impaired ability to revascularise areas of ... concept of therapeutic angiogenesis exploits and supplements the physiological response to hypoxia/ischaemia There have been reports of enhanced ischaemic limb perfusion following injection of bone...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... the time of inclusion, 18 of 44 patients had a SLEDAI score of 0, 17 had a score of and had a SLEDAI score of Exclusion criteria were pregnancy, diabetes mellitus, cancer, presence of cardiovascular ... participant The number of patients and controls included per assay are denoted below Because of the low number of CPCs in the circulation of SLE patients and limitations in the amount of blood to be ... maturation of these cells, the expression of CD133 is lost The combined expression of CD34 and CD133, therefore, defines a population of (immature) circulating progenitor cells Because of the low...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... (T-MPCs) Surface epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal ... speculate that the lower level of expression of particular markers of differentiation could be the result of prior in vivo exposure of T-MPCs to high concentrations of inflammatory cytokines, which ... fetal development is invaded by lymphoid tissue (of mesodermal origin) Therefore, embryologically, tonsils could be a source of MPCs Because of the prevalence of tonsillectomy procedure, tonsils are...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... averages of duplicate cultures The use of lentiviral vectors permitted higher levels of gene transfer (>90%) into CD34+ cells with both of the constructs as assayed by EGFP expression Two rounds of ... http://www.aidsrestherapy.com/content/1/1/2 Figure FACS profiles of thymocyte subsets derived in SCID-hu grafts FACS profiles of thymocyte subsets derived in SCID-hu grafts: To determine the presence of different thymocyte subsets, ... improved level of protection is likely due to higher levels of gene transduction and expression, lower levels of transgene silencing during cell differentiation steps, or a combination of both Although...
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Báo cáo y học: "Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells" pot
... of Nestin and (B) Tuj-1 was determined by Western blot after ethanol treatment Ethanol (50 mM) decreased the expression of Nestin to 70% of control level and increased that of Tuj-1 to 170% of ... Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0016738) Page of 9 10 11 12 13 14 15 16 Author details Department of Pharmacology, College of Pharmacy, ... cycle progression of NPCs culture To determined mechanism of anti-proliferative effect of ethanol, we performed FACS analysis Quantitative graph represented relative proportion of sub G1, S and...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... level of expression of Sall4 isoforms is necessary for normal HSC/HPC activity Moreover, both Sall4A and Sall4B isoforms act as potent regulators of HSC/HPC self-renewal Results Sall4 isoforms ... indicated that overexpression of Sall4 increased the repopulation activity of HSC/HPCs Of note, however, though HSC/HPCs were expanded by sustained ectopic expression of Sall4 isoforms and exhibited enhanced ... contributions of control or Sall4 transduced HSC/HPCs to the cellularities of Yang et al Journal of Hematology & Oncology 2011, 4:38 http://www.jhoonline.org/content/4/1/38 Page of 14 Figure Flow...
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báo cáo khoa học: "Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells" pptx
... was used Excitation power control was 20% Page of remained loosely attached to the bottom of the dish after washing steps The optical spectrum of one of such QD cluster outside EPCs is shown in ... acknowledged Author details Department of Theoretical Chemistry, School of Biotechnology, Royal Institute of Technology, S-106 91 Stockholm, Sweden 2Department of Molecular and Clinical Medicine/Clinical ... shown at 450 nm (i.e., at the edge of the excitation peak which is centred at 458 nm with a FWHM of about 12 nm) in order to be able to show the spatial structure of the sample Strong reflection...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... Formation of type I collagen-producing, alpha smooth muscle actin (aSMA)-positive myofibroblasts is a hallmark of pulmonary fibrosis Despite decades of extensive research, the origin of pulmonary myofibroblasts ... lung tissues differ in their regenerative capability Therefore, we concluded that the microenvironment of injured lung tissue dictates the fate and function of bone- marrow-derived Page 12 of 13 ... one representative out of five independent experiments (A, B) Quantification of flow cytometry analysis of prominin-1 + /CD45+ cells out of all analyzed cells in the lung tissue following bleomycin...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... (University of Konstanz) for their assistance, and Mrs Ina Kähler, Prof M Clauss (University of Indianapolis) and Prof M D Menger (University of Saarland) for the critical reading of the manuscript ... tolerability of intraveinous application of EBM-2 (1 ml) alone turned out to be safe Intravenous application of EPC was performed 50 to 120 after reperfusion of the transplanted left lung Assessment of ... Results Characteristics of ex vivo-generated bone marrow-derived rat EPC Cells were harvested from the non-adherent fraction of rat bone marrow mononuclear cells after 48 h of culture on fibronectin-coated...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... weight) Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias was considered to represent 18.1% of total ... results were shown as percentages of total number of transfused donor cells Histological analysis and detection of donor cells Thin sections (5 µm) of lung tissue were prepared from paraffin-embedded ... frequency of labeled cells was measured by flow cytometry and the total number of labeled donor cells in recipients' bone marrow was calculated The results are shown as percentages of total number of...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... uptake of cell-penetrating dyes [13] or their ability to grow out of cartilage tissue [9] Alternatively, the expression of typical membrane-associated proteins can be employed for the selection of ... the relative percentage of CD166+ chondrocytes by immunofluorescence, analyzing the zonal distribution of these cells within the matrix of normal and OA cartilage was of particular interest Owing ... contributed to acquisition of data, analysis and interpretation of data, and drafting and critical revision of the manuscript SR contributed to acquisition of data and critical revision of the manuscript...
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